Coding

Part:BBa_K2220009

Designed by: Nan Rong   Group: iGEM17_BNU-China   (2017-10-21)


Secrete FliC protein

We add the alpha-factor to the N-terminal of FliC. So it can secrete flagellins with the chasis S.cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1071
    Illegal XhoI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None